Browsing by Author "Pitsouli, Chrysoula"
Now showing items 1-19 of 19
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Article
Developmental biology: Our fly cousins' gut
Pitsouli, Chrysoula; Perrimon, N. (2008)What do we humans have in common with flies? Quite a lot, at least at the cellular and molecular levels. Our intestine, for instance, is similar to that of the fly, not only in function but also in its development and ...
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Article
Embryonic multipotent progenitors remodel the Drosophila airways during metamorphosis
Pitsouli, Chrysoula; Perrimon, N. (2010)Adult structures in holometabolous insects such as Drosophila are generated by groups of imaginal cells dedicated to the formation of different organs. Imaginal cells are specified in the embryo and remain quiescent until ...
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Article
Exploiting position effects and the gypsy retrovirus insulator to engineer precisely expressed transgenes
Markstein, M.; Pitsouli, Chrysoula; Villalta, C.; Celniker, S. E.; Perrimon, N. (2008)A major obstacle to creating precisely expressed transgenes lies in the epigenetic effects of the host chromatin that surrounds them. Here we present a strategy to overcome this problem, employing a Gal4-inducible luciferase ...
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Article
The homeobox transcription factor cut coordinates patterning and growth during drosophila airway remodeling
Pitsouli, Chrysoula; Perrimon, N. (2013)A fundamental question in developmental biology is how tissue growth and patterning are coordinately regulated to generate complex organs with characteristic shapes and sizes. We showed that in the developing primordium ...
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Article
Homeostasis in Infected Epithelia: Stem Cells Take the Lead
Pitsouli, Chrysoula; Apidianakis, Yiorgos; Perrimon, N. (2009)To maintain tissue homeostasis and avoid disease, epithelial cells damaged by pathogens need to be readily replenished, and this is mainly achieved by the activation of stem cells. In this Short Review, we discuss recent ...
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Book Chapter
The Hypoxia-Inducible Factor-1α in Angiogenesis and Cancer: Insights from the Drosophila Model
Tamamouna, Vasilia; Pitsouli, Chrysoula (IntechOpen, 2018)The hypoxia-inducible factor-1α (HIF-1α) is an evolutionarily conserved transcription factor with prominent roles in the hypoxic response, cell survival, angiogenesis and cancer. HIF-1α functions as a sensor of molecular ...
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Article
Immune response to bacteria induces dissemination of Ras-activated Drosophila hindgut cells
Bangi, E.; Pitsouli, Chrysoula; Rahme, L. G.; Cagan, R.; Apidianakis, Yiorgos (2012)Although pathogenic bacteria are suspected contributors to colorectal cancer progression, cancer-promoting bacteria and their mode of action remain largely unknown. Here we report that sustained infection with the human ...
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Article
In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography
Vinegoni, C.; Pitsouli, Chrysoula; Razansky, D.; Perrimon, N.; Ntziachristos, V. (2008)We report a technique for fluorescence tomography that operates beyond the penetration limits of tissue-sectioning fluorescence microscopy. The method uses multi-projection illumination and photon transport description in ...
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Article
The interplay between DSL proteins and ubiquitin ligases in Notch signaling
Pitsouli, Chrysoula; Delidakis, Christos (2005)Lateral inhibition is a pattern refining process that generates single neural precursors from a field of equipotent cells and is mediated via Notch signaling. Of the two Notch ligands Delta and Serrate, only the former was ...
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Article
Intertwined trade-offs coordinate Drosophila midgut mitosis vs. endoreplication and host defense vs. dysplasia
Tamamouna, Vasilia; Panagi, Myrofora; Theophanous, Andria; Demosthenous, Maria; Michail, Maria; Papadopoulou, Markella; Teloni, Savvas; Pitsouli, Chrysoula; Apidianakis, Yiorgos (2019)Inflammatory signaling supports host defense against infection, not only through immune cells, but also via regeneration of damaged tissue. Heightened regeneration, nevertheless, predisposes for all types of cancer and ...
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Article
Intestinal Stem Cells: A Decade of Intensive Research in Drosophila and the Road Ahead
Apidianakis, Yiorgos; Tamamouna, V.; Teloni, S.; Pitsouli, Chrysoula (2017)Since the molecular characterization of Drosophila midgut progenitors in 2006, a few hundred articles studying fly intestinal stem cells have already been published. There was a relative lag phase in creating new knowledge ...
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Article
Mesoscopic fluorescence tomography for in-vivo imaging of developing Drosophila
Vinegoni, C.; Razansky, D.; Pitsouli, Chrysoula; Perrimon, N.; Ntziachristos, V.; Weissleder, R. (2009)Visualizing developing organ formation as well as progession and treatment of disease often heavily relies on the ability to optically interrogate molecular and functional changes in intact living organisms. Most existing ...
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Article
Mesoscopic fluorescence tomography for in-vivo imaging of developing Drosophila.
Vinegoni, C.; Razansky, D.; Pitsouli, Chrysoula; Perrimon, N.; Ntziachristos, V.; Weissleder, R. (2009)Visualizing developing organ formation as well as progession and treatment of disease often heavily relies on the ability to optically interrogate molecular and functional changes in intact living organisms. Most existing ...
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Article
Neuralized Encodes a Peripheral Membrane Protein Involved in Delta Signaling and Endocytosis
Pavlopoulos, Elias; Pitsouli, Chrysoula; Klueg, K. M.; Muskavitch, M. A. T.; Moschonas, Nicholas K.; Delidakis, Christos (2001)Activation of the Notch (N) receptor involves an intracellular proteolytic step triggered by shedding of the extracellular N domain (N-EC) upon ligand interaction. The ligand Dl has been proposed to effect this N-EC shedding ...
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Article
Role of conserved intracellular motifs in Serrate signalling, cis-inhibition and endocytosis
Glittenberg, M.; Pitsouli, Chrysoula; Garvey, C.; Delidakis, Christos; Bray, S. (2006)Notch is the receptor in a signalling pathway that operates in a diverse spectrum of developmental processes. Its ligands (e.g. Serrate) are transmembrane proteins whose signalling competence is regulated by the ...
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Article
Signaling mechanisms controlling cell fate and embryonic patterning
Perrimon, N.; Pitsouli, Chrysoula; Shilo, B. -Z (2012)During development, signaling pathways specify cell fates by activating transcriptional programs in response to extracellular signals. Extensive studies in the past 30 years have revealed that surprisingly fewpathways exist ...
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Article
Synergy between bacterial infection and genetic predisposition in intestinal dysplasia
Apidianakis, Yiorgos; Pitsouli, Chrysoula; Perrimon, N.; Rahme, L. (2009)Accumulating evidence suggests that hyperproliferating intestinal stem cells (SCs) and progenitors drive cancer initiation, maintenance, and metastasis. In addition, chronic inflammation and infection have been increasingly ...
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Article
Tissue communication in regenerative inflammatory signaling: Lessons from the fly gut
Kux, K.; Pitsouli, Chrysoula (2014)The intestine, as a barrier epithelium, serves in the first line of defense against invading pathogens and damaging agents that enter the body via food ingestion. Maintenance of intestinal homeostasis is therefore key to ...
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Article
Unpredictable Effects of the Genetic Background of Transgenic Lines in Physiological Quantitative Traits
Evangelou, Amalia; Ignatiou, Anastasia; Antoniou, Chloi; Kalanidou, Sofia; Chatzimatthaiou, Sotiroula; Shianiou, Gavriella; Ellina, Soteroulla; Athanasiou, Rafaella; Panagi, Myrofora; Apidianakis, Yiorgos; Pitsouli, Chrysoula (2019)Physiology, fitness and disease phenotypes are complex traits exhibiting continuous variation in natural populations. To understand complex trait gene functions transgenic lines of undefined genetic background are often ...